Non-essential shops, bars, gyms hairdressers and other public venues will be closed until at least mid-January. Two guests per household will be allowed - four over the holidays.
Prime Minister Mark Rutte said the measures were "unavoidable".
Countries across Europe have been tightening restrictions as the heavily mutated variant spreads.
The new rules in the Netherlands - the strictest to have been announced over Omicron so far - come into force on Sunday.
"I stand here tonight in a sombre mood. And a lot of people watching will feel that way too," Mr Rutte told a news conference on Saturday. "To sum it up in one sentence, the Netherlands will go back into lockdown from tomorrow."
Booster at least 80% effective against severe Omicron
How can I tell I have Omicron?
Covid map: Where are cases the highest?
Under the new rules, people are being urged to stay at home as much as possible. Strict limits will be placed on the number of people who can meet - a maximum of two guests aged 13 and over will be allowed in people's homes, and four on 24-26 December and on New Year's Eve and New Year's Day.
Events are not permitted other than funerals, weekly markets selling groceries and professional sports matches with no spectators.
All schools will be closed until at least 9 January, while other lockdown measures will remain in place until at least 14 January.
Restaurants can continue to sell takeaway meals, and non-essential shops can offer click and collect services.
The BBC's Anna Holligan in The Hague said the announcement was being met with disbelief and dismay.
"I can now hear the whole of the Netherlands sighing. This is exactly one week before Christmas, another Christmas that is completely different from what we would like," Mr Rutte said.
But, he added, a failure to act now would likely lead to "an unmanageable situation in hospitals".
Earlier on Saturday, people rushed to do their Christmas shopping amid reports that new measures were about to be introduced.
"It's too busy, but I'm coming before the Christmas holidays to pick up gifts, it seems like a new lockdown is coming," Ayman Massori told AFP news agency.
For weeks, curfews have been placed on hospitality and cultural venues in the Netherlands in an effort to limit the spread of Omicron.
The Dutch National Institute for Public Health has reported more than 2.9m coronavirus cases since the pandemic began, and over 20,000 deaths.
It says the Omicron variant currently still accounts for a minority of coronavirus cases in the Netherlands but is spreading rapidly.
Officials say it is expected to become the dominant variant by the New Year.
The head of the Dutch outbreak management team, Jaap van Dissel, said the new measures would "buy time", allowing more people to get booster shots and for the healthcare system to prepare for a possible rise in infections.
"As a country we are best protected if as many people as possible get a booster vaccination," he said.
Media caption,
Watch time-lapse footage showing the long Eurostar queue at London's St Pancras station on Friday evening
France, the Republic of Ireland and Germany have also announced measures designed to curb the infections.
The Omicron variant is "spreading at lightning speed" in Europe and will likely become dominant in France by the start of next year, French Prime Minister Jean Castex has warned.
What are France's new rules for UK travellers?
France has imposed strict travel restrictions on those entering from the United Kingdom - the hardest hit country in the region, with nearly 25,000 confirmed Omicron cases on Saturday.
Europe has already seen more than 89 million cases and 1.5 million Covid-related deaths, according to the latest EU figures.
Non-essential shops, bars, gyms hairdressers and other public venues will be closed until at least mid-January. Two guests per household will be allowed - four over the holidays.
Prime Minister Mark Rutte said the measures were "unavoidable".
Countries across Europe have been tightening restrictions as the heavily mutated variant spreads.
The new rules in the Netherlands - the strictest to have been announced over Omicron so far - come into force on Sunday.
"I stand here tonight in a sombre mood. And a lot of people watching will feel that way too," Mr Rutte told a news conference on Saturday. "To sum it up in one sentence, the Netherlands will go back into lockdown from tomorrow."
Booster at least 80% effective against severe Omicron
How can I tell I have Omicron?
Covid map: Where are cases the highest?
Under the new rules, people are being urged to stay at home as much as possible. Strict limits will be placed on the number of people who can meet - a maximum of two guests aged 13 and over will be allowed in people's homes, and four on 24-26 December and on New Year's Eve and New Year's Day.
Events are not permitted other than funerals, weekly markets selling groceries and professional sports matches with no spectators.
All schools will be closed until at least 9 January, while other lockdown measures will remain in place until at least 14 January.
Restaurants can continue to sell takeaway meals, and non-essential shops can offer click and collect services.
The BBC's Anna Holligan in The Hague said the announcement was being met with disbelief and dismay.
"I can now hear the whole of the Netherlands sighing. This is exactly one week before Christmas, another Christmas that is completely different from what we would like," Mr Rutte said.
But, he added, a failure to act now would likely lead to "an unmanageable situation in hospitals".
Earlier on Saturday, people rushed to do their Christmas shopping amid reports that new measures were about to be introduced.
"It's too busy, but I'm coming before the Christmas holidays to pick up gifts, it seems like a new lockdown is coming," Ayman Massori told AFP news agency.
For weeks, curfews have been placed on hospitality and cultural venues in the Netherlands in an effort to limit the spread of Omicron.
The Dutch National Institute for Public Health has reported more than 2.9m coronavirus cases since the pandemic began, and over 20,000 deaths.
It says the Omicron variant currently still accounts for a minority of coronavirus cases in the Netherlands but is spreading rapidly.
Officials say it is expected to become the dominant variant by the New Year.
The head of the Dutch outbreak management team, Jaap van Dissel, said the new measures would "buy time", allowing more people to get booster shots and for the healthcare system to prepare for a possible rise in infections.
"As a country we are best protected if as many people as possible get a booster vaccination," he said.
Media caption,
Watch time-lapse footage showing the long Eurostar queue at London's St Pancras station on Friday evening
France, the Republic of Ireland and Germany have also announced measures designed to curb the infections.
The Omicron variant is "spreading at lightning speed" in Europe and will likely become dominant in France by the start of next year, French Prime Minister Jean Castex has warned.
What are France's new rules for UK travellers?
France has imposed strict travel restrictions on those entering from the United Kingdom - the hardest hit country in the region, with nearly 25,000 confirmed Omicron cases on Saturday.
Europe has already seen more than 89 million cases and 1.5 million Covid-related deaths, according to the latest EU figures.
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- A partial lockdown has come into force in the Netherlands, amid record Covid cases and concerns over the potentially more infectious new Omicron variant.